This workshop is ideal for trainees who want to advance their biological knowledge and research projects through the use of cutting edge transgenic technologies. The taught techniques are valuable to both human and mouse stem cell research, modifying cells through a transgenic approach. The workshop will be 60% bench work and 40% classroom (talks and in silico analysis). By the end of the workshop trainees will be knowledgeable in designing and generating appropriate expression constructs and CRISPR/Cas9 strategies. The course is tailored so that participants design and generate constructs for their very own research projects.
Skills and techniques taught on the course
● Genome modification by CRISPR mediated gene editing (applicable to knockouts and knock-ins).
● Strategies for generating reporter cell lines. (identifying the genomic region of interest, appropriate fluorescent protein, method of genomic integration and the genomic location).
● Human pluripotent cells culture, transfection, subcloning and genotyping.
● Gene over-expression with transposons in human and mouse stem cells (selecting a promoter, a method of genomic integration and identifying amenable genomic loci).
For more infomration please visit the workshops page.